Supplementary Figure 4: ZEB1, SNAI1 and TWIST1 mRNA levels are not substantially increased in SUM159-P2 cells.
From: ATM-mediated stabilization of ZEB1 promotes DNA damage response and radioresistance through CHK1
(a) qPCR of ZEB1, SNAI1 and TWIST1 in SUM159-P0 and SUM159-P2 cells. n = 3 samples per group. (b) Clonogenic survival assays of U2OS cells transfected with ZEB1 siRNA. n = 3 wells per group. Inset: immunoblotting of ZEB1 and GAPDH. Data in a and b are the mean of biological replicates from a representative experiment, and error bars indicate s.e.m. Statistical significance was determined by a two-tailed, unpaired Students t-test. The experiments were repeated 3 times. The source data can be found in Supplementary Table 3. Uncropped images of blots are shown in Supplementary Fig. 7.
