Supplementary Figure 5: Effect of ZEB1 on CHK1, radiosensitivity and the G2 checkpoint.
From: ATM-mediated stabilization of ZEB1 promotes DNA damage response and radioresistance through CHK1
(a) Immunoblotting of ZEB1, γH2AX, H2AX and GAPDH in ZEB1 shRNA-transduced SUM159-P2 cells with or without ectopic expression of an RNAi-resistant ZEB1 mutant (ZEB1-RE), at the indicated time points after 6 Gy IR. (b) Clonogenic survival assays of ZEB1 shRNA-transduced SUM159-P2 cells with or without ectopic expression of an RNAi-resistant mutant (ZEB1-RE). n = 3 wells per group. (c) Percentage of the G2/M population. SUM159-P2 cells were transduced with ZEB1 shRNA, treated with 6-Gy IR and analysed by flow cytometry. n = 3 wells per group. Data in b and c are the mean of biological replicates from a representative experiment, and error bars indicate s.e.m. Statistical significance was determined by a two-tailed, unpaired Students t-test. The experiments were repeated 3 times. The source data can be found in Supplementary Table 3. Uncropped images of blots are shown in Supplementary Fig. 7.
