Supplementary Figure 7: ATR does not regulate ZEB1.
From: ATM-mediated stabilization of ZEB1 promotes DNA damage response and radioresistance through CHK1
(a) 293T cells were transfected with SFB–ZEB1, followed by pull-down with streptavidin-sepharose beads (s-s beads) and immunoblotting with antibodies to ATR and ATM. (b) SUM159-P2 cells were transfected with ATR siRNA and treated with IR. Lysates were immunoblotted with antibodies to p-ATR, ATR, ZEB1 and GAPDH. (c) SUM159-P2 cells were pretreated with ETP-46464 and treated with IR. Lysates were immunoblotted with antibodies to ZEB1, p-CHK1, CHK1, p-CHK2, CHK2 and GAPDH.
