Supplementary Figure 8: ATM-dependent phosphorylation of ZEB1 at S585 is critical for radiation-induced stabilization of ZEB1 but not the interaction between ZEB1 and USP7.
From: ATM-mediated stabilization of ZEB1 promotes DNA damage response and radioresistance through CHK1
(a) 293T cells were transfected with SFB–ZEB1 (wild-type, S585A or S585D), followed by pull-down with streptavidin-sepharose beads (s-s beads) and immunoblotting with the USP7 antibody. (b,c) Quantification of ZEB1 proteins levels (normalized to co-transfected GFP) in Fig. 7i. Uncropped images of blots are shown in Supplementary Fig. 7.
