Supplementary Figure 4: Late Mesp1 progenitors also contribute to the head. | Nature Cell Biology

Supplementary Figure 4: Late Mesp1 progenitors also contribute to the head.

From: Early lineage restriction in temporally distinct populations of Mesp1 progenitors during mammalian heart development

Supplementary Figure 4

(a–a’) Example of a Mesp1-rtTA/TetO-Cre/Rosa-Confetti embryo with co-labelled head (a) and heart (a’) at E12.5. Scale bars, 200 μm. (b–b’) Sections of a Mesp1-rtTA/TetO-Cre/Rosa-Confetti labelled embryo showing labelling in the head in an extraocular muscle (EOM) (b) as well as in the right ventricle RV (b’). Scale bars, 200 μm. (c) Temporal appearance of head muscle labelling inferred from all datasets (n = 105 independent embryos translating to n = 181 embryos by colour). Plotted is the fraction of head muscle labelling induced at each induction time for a given colour. Head muscles are preferentially labelled at the late time points. (d) Regional contribution of head progenitors in monoclonal datasets (n = 5), showing the co-labelling of the head with the heart and preferentially SHF derivatives or the right ventricle (RV). OFT, outflow tract; RV, right ventricle; LV, left ventricle; RA, right atrium; LA, left atrium. Errors bars indicate one sigma confidence intervals.

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