Supplementary Figure 1: Mesp1-rtTA/tetO-Cre transgenic mice induced Cre expression similarly to Mesp1-Cre Knock-in mice. | Nature Cell Biology

Supplementary Figure 1: Mesp1-rtTA/tetO-Cre transgenic mice induced Cre expression similarly to Mesp1-Cre Knock-in mice.

From: Early lineage restriction in temporally distinct populations of Mesp1 progenitors during mammalian heart development

Supplementary Figure 1

(a,b) Sections of E12.5 Mesp1-Cre/Rosa-tdTomato (a) and Mesp1-rtTA/TetO-Cre/Rosa-tdTomato hearts (induced by Dox administration between E6.25 and E7.5) (b) and co-stained with DAPI. Both transgenic hearts have a similar expression of the tdTomato with a negative region in the OFT that derive from Mesp1 negative neural crest cells (asterisks). c–e Doxycycline injection has no effect on Mesp1 expression during early mouse embryonic development. (c–d) In situ hybridization for Mesp1 expression in early embryo at E6.5. The detection of Mesp1 mRNA in the primitive streak (PS) and the nascent lateral mesoderm is similar in embryo that did not receive DOX (c) and in embryos injected with DOX (+ DOX) (d). A, anterior; P, posterior. e Expression of Mesp1 analysed by RT-qPCR in early embryos (E6.75) without (n = 9) or after doxycycline injections (n = 6). These data show no difference in Mesp1 expression after DOX injection. (f–g) In situ hybridization for Cre expression in early embryos at E6.75. The detection of Cre mRNA in the primitive streak (PS) and the nascent lateral mesoderm is similar in Mesp1-Cre knock-in (f) and in Mesp1-rtTA/TetO-Cre transgenic embryos injected with DOX at E6.25 (+ DOX) (g). Cre expression is similar to the endogenous Mesp1 expression in wild type embryos (h). A, anterior; P, posterior. Scale bar, 500 μm.

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