Supplementary Figure 1: lkt/abcc4-deficient embryos display randomization of organ laterality.

(a) cmlc2 expression showing the laterality (left-jagging versus right-jagging) of heart tube in wild-type (wt) embryos and lkt mutants. (b) foxA3 expression depicting the sidedness of liver (black arrow) and pancreas (white arrow) in wt embryos and lkt mutants. (c) insulin expression displaying the pancreas laterality in wt embryos and lkt mutants. (d) lft1 expression showing brain laterality in wt embryos and lkt mutants. (e,f) Acetylated tubulin immunostaining reveals normal pronepheric cilia development in lkt mutants compared with wild-type embryos. Scale bar: 100 μm (a–f); 10 μm (e,f). (g,h) Analysis of sidedness of asymmetric gene expression in wt embryos, lkt mutants and lkt/abcc4 morphant embryos. The total numbers of embryos analysed pooled from n = 3 independent experiments are indicated on the top of each bar. The number of embryos with sided expression of each marker divided by total number of embryos. The percentages of embryos with LR defects were significantly higher in lkt mutants or abcc4 morphants as compared with wt embryos, but not statistically different among lkt mutants or abcc4 morphant embryos (Yates corrected Chi-square test, ^∗∗∗P < 0.0001, one degree of freedom). Statistics source data for Supplementary Fig. 1g, h can be found in Supplementary Table 2.