Supplementary Figure 5: Deletion of RASSF1A/LATS1 axis compromises fork recovery after stress. | Nature Cell Biology

Supplementary Figure 5: Deletion of RASSF1A/LATS1 axis compromises fork recovery after stress.

From: RASSF1A–LATS1 signalling stabilizes replication forks by restricting CDK2-mediated phosphorylation of BRCA2

Supplementary Figure 5

(a) IdU tract length distributions from DNA fibres of Lats1+/+, Lats1−/− and Lats1−/−mycLATS1 after release from 4 h treatment with 2 mM HU, to determine the ability of the cells to recover after replication stress. (b) IdU tract length distributions of Rassf1A+/+, Rassf1A−/−, Rassf1A−/−FLAG−R1A and Rassf1A−/−FLAG−R1A−A133S MEFs after release from 2 mM HU. 100 tracks were analysed in each condition in n = 3 independent experiments. Statistical significance was determined by a two-tailed t-test. Bar-graphs derived from a representative experiment. 100 tracks were analysed per condition. Mean track lengths and standard deviation given in parenthesis derived from n = 3 independent experiments. Statistical significance was determined by a two-tailed t-test. Sketch delineates experimental design.

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