Supplementary Figure 3: Endothelial podosome markers and endothelial density in tumors vessels.
From: Endothelial podosome rosettes regulate vascular branching in tumour angiogenesis
(a) Confocal images of representative vessels in angiogenic islets of RipTag2 mice. Xyz-section of immunostaining for primary Abs as indicated and nuclear-stained by DAPI (blue). Vessels are delimited by white dotted lines; white arrows indicate podosome-rosettes. Scale bar, 3 μm. (b) 3D isosurface rendering of tumor vessels in a 12-μm-thick slice of a representative RipTag2 angiogenic islet. Vessels (gray) detected with laminin staining and podosome-rosettes (red) recognized with colocalization of F-actin/cortactin staining. Red arrows indicate podosome-rosettes. Tickmarks on axis: 10 μm. (c) Representative micrograph images of CD31 and VEGF staining in biopsy samples of lung tumors. Scale bar, 50 μm. Quantifications and correlations in Fig. 2D.
