Supplementary Figure 1: Endothelial podosome rosettes in cultured EC and aortic explants.
From: Endothelial podosome rosettes regulate vascular branching in tumour angiogenesis
(a–b) Immunostained representative VEGF-A-stimulated EC treated with PMA for 30 min. Scale bar, 10 μm. (c) Cytofluorimetric analysis of membrane MT1-MMP localization. EC treated with PMA for the indicated time. Normalized mean ± SEM of n = 3 independent experiments in which 8 × 104 cells were analyzed per experimental point. (∗∗, P < 0.01 versus T = 0). Statistical significance was calculated using paired nonparametric Wilconox test. (d) Schematic representation of mouse aortic explant anatomy. Endothelial cells (EC) are characterized by large round nuclei and vascular smooth muscle cells (SMC) by thin and elongated nuclei. Along the z-axis, the two cell types were seen separated by the top elastic lamina. Yellow dotted line, schematization of the z plane of microscopic analysis of endothelial rosettes. (e) Immunostaining of a representative 48h VEGF-A-stimulated aortic explant. In yellow, 3D reconstruction of the colocalization channel in podosome rosette. Individual channel images from Fig. 1e. Scale bar, 20 μm.
