Supplementary Figure 5: Analysis of Ambra1 levels, cell proliferation and autophagy in Ambra1 +/gt mice. | Nature Cell Biology

Supplementary Figure 5: Analysis of Ambra1 levels, cell proliferation and autophagy in Ambra1 +/gt mice.

From: AMBRA1 links autophagy to cell proliferation and tumorigenesis by promoting c-Myc dephosphorylation and degradation

Supplementary Figure 5

(a) Analysis of Ambra1-mRNA levels in tissues of, wild-type (+/+) and heterozygous (+/gt) 3-months old Ambra1 mice. Data are presented as means ± s.e.m. and significance is ∗∗P < 0.005 (n = 3 mice per each genotype). (b) We extracted proteins from lung, liver and kidney organs of wild-type or heterozygous for Ambra1 3-months old mice. Ambra1 levels were analysed by western blot using an anti-Ambra1 and anti-Actin antibody. (c) Analysis of Ambra1 levels in lung and liver organs from wild-type (Ambra1 +/+) or heterozygous (Ambra1 +/gt) mice. We selected both tumour tissue and the surrounding healthy tissue from the same animal. Ambra1 protein levels were monitored by western blot, using an anti Ambra1 antibody. (d) p62 immunostaining on liver of Ambra1 heterozygous (Ambra1 +/gt) mice. T: tumour. Scale bar, 100 μm. (e) Western blot analysis of p62 in liver of Ambra1 heterozygous (Ambra1 +/gt) mice. Both tumour tissue and the surrounding healthy tissue were analysed from the same animal.

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