Supplementary Figure 3
From: White-to-brown metabolic conversion of human adipocytes by JAK inhibition
(a) UCP1 expression is increased in PSC-WA after treatment with indicated compounds. Rosiglitazone induced expression of both UCP1 and FABP4. Values represent the mean of two biological replicates. (b) Browning screen performance: Plate-wise control distribution and Z’ factors for UCP1 and FABP4. For UCP1, <Z’ >= 0.50, for FABP4, <Z’ >= 0.64. (c) Reproducibility of replicates. The correlation of the intra-plate replicates for UCP1 (left) and FABP4 (right) in log–log representation. Controls are plotted in red (negative) and green (positive). Compounds with large inter-run differences were excluded from further analysis. (d) Hit selection. Left panel: Quantile–quantile plot of the normalized UCP1 reads. The blue line indicates the expected profile for a Gaussian distribution without actives. The blue dashed lines limit the confidence band of a correlation test corroborating the hit selection from a different angle, as all the selected compounds (green dots) lie outside. The P-value distribution drawn in the inset, which is the basis of the hit selection, shows no irregular features. Right panel: Scatter plot of the normalized FABP4 and UCP1 signal on logarithmic axes. Black dots: inactive compounds. Color dots: active compounds, red: rosiglitazone, yellow: rosiglitazone-like compounds in screen, blue: potential browning hits in screen, green: rosiglitazone-like compounds and browning hits confirmed in validation run, grey : non-confirmed in validation run.
