Supplementary Figure 8: Assessment of GR and VDR target genes.

(a) Q-PCR validation of coordinately up-regulated genes. Cells were treated with Dex (blue) or TNF (red) for the indicated lengths of time before RNA harvest (data represent mean of n = 3 dishes, data from 1 additional independent experiment shown in Source Data Table). (b) Expression of up-regulated target genes in cells transduced with lentivirus carrying shRNA directed against VDR or GR (vs. scrambled control: shScr) and then treated with Dex (D) or TNF (T) for 6 days (data represent mean of n = 3 dishes, data from 1 additional independent experiment shown in Source Data Table). (c) Expression of coordinately up-regulated genes was measured in white adipose tissue samples from chow and high fat-fed C57Bl/6 mice (n = 6 mice P < 0.05, Student’s t-test, mean ± SEM). (d) Expression of coordinately up-regulated genes was measured in white adipose tissue samples from ob/+ and ob/ob mice, treated with vehicle or rosiglitazone for 6 weeks (n = 7 for ob/+, n = 8 for ob/+ plus Rosi, n = 7 for ob/ob with and without Rosi, P < 0.05, Student’s t-test, mean ± SEM).