Supplementary Figure 3
(A,B) Surface points in control (A) and blebbistatin (B) treated cells can be classified into different categories (coloured highlights) by thresholding the Mean and Gaussian curvature. (C,D). This permits comparison of the fraction of surface mesh triangles in each category between experimental conditions (Fig. 1). (E–H) Local surface curvature versus F-actin at the cortex. (E) 2D histogram of maximum absolute curvature versus F-actin intensity via phalloidin stain for an example cell (shown in F). The colours represent log10 of the surface points counted, as in Fig. 3. Green box indicates region of voxels selected with a given range of curvature and F-actin intensity. These are then mapped back to the cell surface (F, green). Similarly, a higher range of F-actin intensities at a higher range of curvature values (G) can be mapped onto the surface (H, green), highlighting predominantly tip structures.
