Supplementary Figure 5: Membrane localized Td-Tomato-CAAX does not show curvature-dependence in cells confined in agarose chambers. | Nature Cell Biology

Supplementary Figure 5: Membrane localized Td-Tomato-CAAX does not show curvature-dependence in cells confined in agarose chambers.

From: Myosin II controls cellular branching morphogenesis and migration in three dimensions by minimizing cell-surface curvature

Supplementary Figure 5

Endothelial cells confined in agarose coffins were imaged and registered to obtain average positional intensity, as described in main text and Methods. (A) Normalized mean intensity of GFP-myosin IIA (green), Td-Tomato-CAAX (purple) and calculated mean curvature as a function of distance along the confined borders of cells as shown in Fig. 4. CAAX signal shows some fluctuations, but are not correlated with curvature. (B) Probability distribution of mean curvature versus mean Td-Tomato-CAAX for all points along confined cell boundaries. n = 6 cells, 95126 points. Spearman’s ρ = −0.093, p = 8.8 × 10−164. Solid white line indicates mean and dashed lines indicate bootstrapped 95% confidence intervals.

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