Supplementary Figure 8: USP30 deubiquitinates outer membrane mitochondrial substrates.
From: USP30 and parkin homeostatically regulate atypical ubiquitin chains on mitochondria
(A) Western blot confirmation of USP30 induction following doxycycline administration and mitochondrial depolarization upon CCCP treatment. USP30 showed strong induction, while reduced MFN1 levels and increase in Opa1 processing were observed. Representative Western blot shown from experiment 1. (B,C) Volcano plots of ubiquitinated proteins identified in Experiments 1 and 2, respectively. The fold change of ubiquitination for each protein between Combo (USP30 overexpression and CCCP) versus CCCP is denoted on the x-axis with significance reported on the y-axis. Both values were determined based on the aggregate values of individual KGG peptides using LiME analysis. Proteins with p-value < 0.01 are labelled; mitochondria-associated proteins are colored red.
