Supplementary Figure 2: 2-DG does not affect MST phosphorylation.
From: Cellular energy stress induces AMPK-mediated regulation of YAP and the Hippo pathway
(a) Effect of MST overexpression on 2-DG induced YAP phosphorylation. HEK293A cells were transiently co-transfected with indicated plasmids. After transfection, cells were treated with 25 mM 2-DG for 1 h and YAP phosphorylation status was determined by phos-tag gel. (b) 2-DG does not affect MST1 phosphorylation. Endogenous MST1 was immunoprecipitated from control or 2-DG (25 mM, 2 h) treated HEK293A cells. MST1 phosphorylation was detected by a phospho-MST (T183) specific antibody.
