Supplementary Figure 1: Phylogenetic analysis of Syndetin orthologs.
From: EARP is a multisubunit tethering complex involved in endocytic recycling
An initial BLASTP search revealed that the first 492 amino acids of human Syndetin comprised the largest conserved domain (DUF 2450). This sequence was used in two consecutive rounds of PSI-BLAST on genomes from different organisms. Orthologs were defined as (i) being large proteins (>500 amino acids in all super-groups, with the exception of some smaller proteins in the SAR/CCTH super-group), (ii) having a high α-helical content, (iii) having either DUF 2450 or DUF 2451 domains, and (iv) yielding human Syndetin and not Vps54 as one of three top hits in reverse PSI-BLAST searches. Listed are representative species, NCBI reference sequences and length of the Syndetin orthologs.
