Supplementary Figure 5: Actin distribution and mechanical properties of the cytoplasm.

a: No differences in actin density are detected across the cytoplasm of Fmn2−/− oocytes injected with Formin 2. Actin densities are represented as the ratio of GFP-UtrCH integrated fluorescence intensities of cytoplasmic regions near the cortex, in the middle and near the nucleus normalized by the region area over integrated fluorescence intensity of the entire oocyte normalized by the oocyte surface. P-value for one-way ANOVA = 0.48. n = 8 oocytes. Error bars display SEM. b: No density gradient of actin vesicles is observed in Fmn2−/− oocytes injected with Formin 2. Actin vesicles density as a function of the distance from the cortex. Densities are represented as a ratio with the highest density observed in the area 10–15 μm away from the cortex (relative density of 1). n = 8 oocytes. c: Direct measurements by optical trapping of mechanical properties in different regions of the cytoplasm in wt oocytes. Elastic (G′) and viscous (G′′) moduli are measured in Pa at 10 Hz. Mean and SD for elastic moduli are 4.6 ± 2.2 near cortex, 4.6 ± 2.3 in the middle, 4.3 ± 1.8 near nucleus. Mean and SD for viscous moduli are 4.9 ± 2.8 near cortex, 4.3 ± 1.7 in the middle, 4.0 ± 1.5 near nucleus. P-values for Kolmogorov–Smirnov test for equality of distributions are 0.83 for cortex-middle, 0.98 for cortex-nucleus, 0.91 for middle-nucleus (elastic moduli); 0.98 for cortex-middle, 0.98 for cortex-nucleus, 0.72 for middle-nucleus (viscous moduli). n = 32 vesicles measured in 11 oocytes Data are aggregated from five independent experiments in a and b and from three independent experiments in c.