Supplementary Figure 4: Centromere assembly failure occurs not only in meiocytes with functional spindles, but also in those with dysfunctional spindles.

Series of frames from films of cells undergoing meiosis. Cnp1 and Swi6 are observed as in Fig. 2. Histone H3 is observed by CFP tagging as in Supplementary Fig. 1, and tubulin and SPB by endogenous mCherry tagging of Atb2 and Sid4. Numbers below frames represent minutes before or after metaphase I. Scale bars represent 5 μm. In these three examples, SPBs are scattered far from chromatin and spindles fail to assemble properly. Some chromosomes lacking kinetochore signals (either Cnp1 or Swi6; arrows) can be discerned as they separate from the main chromatin mass.