Supplementary Figure 6: Impaired kinetochore function in meiocytes harbouring circular chromosomes.
From: The telomere bouquet regulates meiotic centromere assembly
Series of frames of cells undergoing meiosis. Cnp1, tubulin, SPB and chromatin are observed via ectopically expressed Cnp1-GFP, Atb2-mCherry, Sid4-mCherry (red) and Hht1-CFP, respectively. Numbers below frames represent minutes before or after metaphase I. Scale bars represent 5 μm. Circular chromosome-containing meiocytes suffer similar phenotypes to bouquet-deficient linear chromosome-containing meiocytes. In these examples, spindles form properly but some chromosomes (arrows) lack kinetochore signals and fail to attach. The top two series show MI missegregation, and the bottom shows an unsegregated chromatid at MII.
