Supplementary Figure 1: Bouquet deficient meiocytes show failure of chromosome attachment to correctly formed spindles.

(A–C) Examples of bouquet-deficient cells undergoing meiosis. Tubulin and histone H3 are observed via ectopically expressed GFP-Atb2 (green) and endogenous mRFP tagging of one of the two alleles encoding Hht1 (red), respectively. Numbers below frames represent minutes before or after metaphase I. Scale bars represent 5 μm. (D,E) Tubulin and histone H3 are observed via endogenously tagged Atb2-mRFP (red) and endogenous CFP tagging of one of the two alleles encoding Hht1 (blue), respectively. Labels as in A. (A,B,D,E) bqt1Δ meiosis. The spindle forms correctly but some chromosomes (arrows) fail to attach to those spindles and remain unsegregated. (C) rap1Δ meiosis. The spindle forms correctly but some chromosomes (arrows) fail to attach to those spindles and remain unsegregated. (F) Bouquet deficient cells form spindles with normal elongation rates. The time from anaphase I onset to the moment of maximal spindle length was measured. Red dots represent values for each individual cell; black lines represent the mean +/− standard error. Wt and bqt1Δ cells with good spindles have identical maximal spindle lengths and rates of spindle elongation.