Supplementary Figure 5: mRNA and protein expression levels after siRNA transfections.
From: Control of cell–cell forces and collective cell dynamics by the intercellular adhesome
(a) Scheme illustrating the localization of all targeted proteins. (b) Levels of mRNA after 5 days of transfection. Data were quantified by RT-PCR. Data are presented as mean ± SEM (normalized to control cells). RT-PCR was run in triplicate. n = 12 samples pooled from 4 independent experiments for CT siRNA, Ncad siRNA, Pcad siRNA, DSC3 siRNA, αcat siRNA, p120 siRNA, LIMA1 siRNA, CLDN8 siRNA, JAM-A siRNA; n = 9 samples pooled from 3 independent experiments for βcat siRNA, DDR1 siRNA, VCL siRNA, CLDN1 siRNA, CLDN7 siRNA, ZO-1 siRNA, ZO-3 siRNA, PKP2 siRNA, JUP siRNA; n = 6 samples pooled from 2 independent experiments for CLDN4 siRNA, CX43 siRNA, OCLN siRNA. (c) Protein levels after 5 days of transfection. Data are presented as mean ± SEM. For each protein, n = 3 samples pooled from 3 independent transfections (normalized to control cells).
