Supplementary Figure 6: The effect of SR12-GFP α-actinin-1 fragment on actin cytoskeleton self-organization.
From: Cellular chirality arising from the self-organization of the actin cytoskeleton
(a,b) Actin organization (visualized by tdTomato-F-Tractin) in SR12-GFP expressing cells and high magnification time-series images illustrating actin dynamics (white-boxed region). 60% of SR12-GFP transfected cells demonstrated phenotypes similar to control cells (30% counter-clockwise swirling and 30% non-swirling). The remaining 40% of the transfected cells were found to express a higher level (2-fold on average) of SR12-GFP. 60% of these higher SR12-GFP expressing cells failed to progress beyond the circular stage (a) while the remaining 40% exhibited similar behavior to GFP-ABDdel-α-actinin-1 mutant expressing cells, forming characteristic “single-ended” stress-fibers that tilt in a positive direction (b). An example of two “single-ended” stress-fibers are shown here (white and cyan arrowheads). Scale bars, 10 μm; 5 μm (high-magnification). n = 39 transfected cells pooled from 2 independent experiments.
