Supplementary Figure 7: Phospho Myosin light chain localization in selective junctions is not reflected in Par3, PDZ RhoGef (AHRGef11) or phospho-Ezrin localization. | Nature Cell Biology

Supplementary Figure 7: Phospho Myosin light chain localization in selective junctions is not reflected in Par3, PDZ RhoGef (AHRGef11) or phospho-Ezrin localization.

From: Myosin-II-mediated cell shape changes and cell intercalation contribute to primitive streak formation

Supplementary Figure 7

(a) Phospho-Myosin regulatory light chain shows only partial co-localization with Myosin IIb, (similar results for IIa are not shown), suggesting that only a fraction of Myosin IIb is assembled into mini-filaments in apical junctions. (b) Phospho-Myosin light chain is only found in selective junctions, which are organised in a super cellular pattern in mesendoderm cells. (c) Par3, PDZRhoGef (AHRgef11) and phospho-Ezrin do not show an asymmetric cellular localization in mesendoderm cells. The figures are representative of at least 2 experiments per antibody typically between 10 embryos /experiment. White scale bar 25 μm.

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