Supplementary Figure 4
From: Anisotropic stress orients remodelling of mammalian limb bud ectoderm
(a) Length-width ratio of control βcatf/f and βcatf/f; Crect mutant limb bud ectodermal cells. (n = 3 19–21 som. embryos per condition; p = 0.0324 (Student’s t-test)) (b) Angle of cell orientation in control βcatf/f and βcatf/f;Crect mutant limb bud ectoderm. Histogram shows distribution of cells grouped into 6 bins of 15° (n = 3 19–21 som. embryos per condition; p = 0.0332 (75–90° bin) (Student’s t-test)). (c) Time series of initiating limb bud ectoderm cells in WT and βcatf/f; Crect mutant embryos expressing R26R::Venus-Actin. (d, e) Representative force curves for control βcatf/f (d) and βcatf/f; Crect (e) mutant AFM experiments. (f) Confocal XY section of control βcatf/f and βcatf/f;Crect mutant limb bud ectoderm visualizing E-cadherin (green) and DAPI (blue). (g) Axin2 expression in embryos that were treated in 6-hour roller culture with DMSO control (top) or IWR-1 (bottom). (h) phospho-Dishevelled 2 (p-Dvl2) is diminished in embryos treated with IWP-2. Uncropped blots in Supplementary Fig. 6. Scale bar indicates 20 μm (c, f), 200 μm (g); anterior is left and ventral is up. Error bars indicate SEM. Asterisk indicates P < 0.05.
