Supplementary Figure 5: Effects of LatB treatment on DC.

Time point 0 sec is within 5–10 sec of injection with the various LatB concentrations (2 independent experiments, 18 embryos were injected with 100 mM LatB, 18 embryos with 10 mM, and 5 embryos with 1 mM). In each experiment, the second frame was chosen to depict the time point when the first defects are evident meaning that the known actin-based force generating systems, the AS tissue and the actin/MyoII purse string begin losing tension and integrity. Red arrows depict rupturing points. The following frames show the timeline of the resulting DC area disintegration. The third frame depicts the time point right before first defects of the zipping area become evident. The fourth frame the time point when also the zipping area is clearly disrupted. The LatB effect is scalable—the higher the concentration, the faster rupturing of the tissues occurs. However, common to all LatB concentrations is that the zipping area only starts showing defects with a considerable time delay, suggesting that this force generating process does not react acutely to actin filament depolymerization, unless for some reason LatB mediated actin depolymerization is less efficient in zipping LE cells as compared to LE cells before or post zipping.