Supplementary Figure 2: Human pancreatic MPC enhancers.
From: TEAD and YAP regulate the enhancer network of human embryonic pancreatic progenitors
(a) Examples showing how in vitro MPCs recapitulate the epigenomic landscape of in vivo MPCs. HNF1B encodes a TF that important for pancreas development, FZD2 is a non-canonical WNT signaling component, and HES1 is a transcriptional repressor that controls growth and differentiation of pancreatic MPCs. (b) Enhancers were defined as H3K27ac islands in the in vitro MPCs that overlapped H3K4me1 islands in both in vitro and in vivo MPCs. We discarded regions overlapping promoters (1 Kb upstream and 2 Kb downstream of RefSeq TSS) and any regions smaller than 50 bp. This revealed 9,669 MPC enhancers. (c) MPC enhancers are tissue- and stage-selective. Enhancers were defined for 8 tissues in a similar manner to MPCs (Supplementary Table 8). Each pie chart shows in red the proportion of MPC enhancers that are inactive in each tissue. We defined MPC-selective enhancers as those that were inactive in at least 6 out of 7 non-pancreatic tissues. (d) Enriched annotated functions among genes that are associated with three or more MPC-selective enhancers. The graph shows fold enrichment values and P values calculated with GREAT53.
