Supplementary Figure 6: Developmental expression of YAP.
From: TEAD and YAP regulate the enhancer network of human embryonic pancreatic progenitors
(a) Immunofluorescence images of hESCs and different stages of differentiation show that YAP is strongly expressed in the nuclei of hESCs (white arrows), whereas a marked decrease in YAP immunoreactivity is observed in definitive endoderm and dorsal foregut stages (days 3 and 6)(white arrowheads). In days 3 and 6, YAP is not detected in a subset of SOX17+ and FOXA2+ cells, respectively (hollow arrowheads). (b) YAP is present in the nuclei of dorsal foregut endoderm cells of human Carnegie Stage 10 embryos. AIP: anterior intestinal portal, fg: foregut, lm: lateral mesoderm, nc: notochord, nt: neural tube. (c) Immunofluorescence images of mouse E10.5 and E12.5 embryonic pancreas show Tead1 and Yap expression in most nuclei of Pdx1 + MPCs (white arrows) and in the surrounding mesenchyme. Yap expression is absent in glucagon-expressing endocrine cells (hollow arrowheads). The squares in the leftmost panels depict areas shown at higher power in other panels. du: duodenum, dp: dorsal pancreas. (d,e) Yap is broadly expressed in the nuclei of pancreatic mesenchyme and epithelium from E12.5 and E14.5 mouse embryonic pancreas, yet shows cytoplasmic localization in Cpa1 + progenitor cells (white arrowheads) and is undetectable in early Pax6 + endocrine cells (hollow arrowheads). (f) In the adult mouse pancreas Yap is present in nuclei from ducts (white arrows), and not in endocrine (hollow arrowheads) or acinar cells (white arrowheads). (g) YAP is expressed in nuclei of SOX9 + epithelial cells but absent in insulin-expressing endocrine cells from 14 weeks post-conception (WPC) human pancreas. (h) PDX1 co-stains with YAP and TEAD1 in the nucleus of in vitro MPCs. (i) Nuclear YAP is not detected in differentiated insulin-expressing cells derived from hESCs (hollow arrowheads).
