Supplementary Figure 7: Knockdown of Yap1 or dominant inhibition of Tead reduces pancreas size in zebrafish.
From: TEAD and YAP regulate the enhancer network of human embryonic pancreatic progenitors
(a) Injection of a morpholino targeting yap1 (Mo-yap1) or mRNA encoding a TEAD protein fused to the transcriptional repressor domain of Engrailed (TEAD-EnR) decreased the number of insulin expressing cells detected by in situ hybridization. This phenotype was rescued by co-injection of Mo-yap1 with an in vitro synthesized yap1 mRNA that is not sensitive to morpholino inhibition. The percentage of embryos from each condition showing reduced insulin-positive cells was quantified as an indication of pancreatic hypoplasia, and displayed in the graph shown on the side (n = 46-71 embryos per condition). Scale bar = 0.25 mm. (b) Mo-yap1 increased ectopic expression of pancreatic markers. The panels show insulin in situ hybridization in control and Morpholino-treated 24 hpf zebrafish embryos. Scale bar = 0.25 mm.
