Supplementary Figure 3: PLIN2 and PLIN3 associate with hsc70.

(a) Coimmunostaining for PLIN3 and hsc70 in CTR and L2A(−) cells treated or not with OL. Colocalized pixels are in white. Boxed areas are shown at higher magnification. Bottom: graph: percentage colocalization of PLIN3 (bottom) with hsc70. n = 5 independent experiments with 40 cells per condition in each experiment. (b) Coimmunostaining for PLIN2 and hsc70 in CTR and L2A(−) cells treated as indicated. Colocalized pixels are in white. Graph: percentage colocalization. n = 4 independent experiments with 40 cells per condition in each experiment. (c,d) Immunoblot for indicated proteins of immunoprecipitates (IP) of hsc70 from total extracts of CTR and L2A(−) cells treated or not with OL. Representative blots of n = 3 independent experiments. (e) Immunoblot for indicated proteins of IP of GAPDH (top) and IkB (bottom) from total extracts of CTR and L2A(−) cells. (f) Immunoblot for indicated proteins of IP of PLIN2 (top) and PLIN3 (bottom) from total extracts of CTR and L2A(−) cells treated with the indicated increasing concentrations of OL. These blots are an extension of those shown in Fig. 3e, f to show the dose-dependence effect. Representative blots of n = 3 (PLIN3) and 4 (PLIN2) independent experiments. Values are mean ± SEM. Differences are significant for ^∗P < 0.05,^∗∗P < 0.01,^∗∗∗P < 0.001 using Student’s t-test. Uncropped images of blots are shown in Supplementary Fig. 8. Source data is available in Supplementary Table 1.