Supplementary Figure 6: Failure to remove PLINs by CMA alters association of macroautophagy proteins with LD. | Nature Cell Biology

Supplementary Figure 6: Failure to remove PLINs by CMA alters association of macroautophagy proteins with LD.

From: Degradation of lipid droplet-associated proteins by chaperone-mediated autophagy facilitates lipolysis

Supplementary Figure 6

(a,b) Costaining for BODIPY 493/503 and the indicated macroautophagy proteins in CTR cells treated or not with OL. Colocalized pixels are in white. Graph: Percentage colocalization with BODIPY. n = 3 independent experiments with 40 cells per condition in each experiment. (c–f) Costaining for BODIPY493/503 and indicated macroautophagy proteins in CTR and L2A(−) cells treated with OL. Right in c: Colocalized pixels of the boxed area at higher magnification. e,f: Colocalized pixels are shown. Graphs: percentage colocalization with BODIPY. n = 6 (d), 7 (e), 3 (f) independent experiments with 30 (d) 40 (e), 10 (f) cells per condition in each experiment. Values are mean ± SEM. Differences are significant for ∗P < 0.05,∗∗P < 0.01,∗∗∗P < 0.001 using Student’s t-test. Source data is available in Supplementary Table 1.

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