Supplementary Figure 5: Comparison of GFP-PAC-1N in hmp-2(RNAi) and hmp-1(RNAi) embryos.

(a–c) GFP-PAC-1N in live embryos of the indicated genotype; images were captured at the same exposure. (d) Quantification of GFP-PAC-1N contact enrichment. Circles represent data points from individual embryos, and the average is a gray line. ∗∗p < 0.01; n.s. = not significantly different, Mann–Whitney U test. (Control n = 13 embryos, hmp-2(RNAi) n = 9, hmp-1(RNAi) n = 9). Scale bars, 10 μm.