Supplementary Figure 6: Genetic requirements for catenin localization.

Control embryos are wild-type embryos fed on bacteria containing empty RNAi vector. (a,b) HMP-1 immunostaining at cell contacts in control embryos (40/40 embryos) or in the cytoplasm of hmp-2(RNAi) embryos (35/35 embryos). (c,d) α-JAC-1 antiserum immunostains cell contacts in wild-type embryos (30/30 embryos) but not in jac-1(xn15) embryos (42/42 embryos). α-JAC-1 antiserum also stains nuclei non-specifically, as evidenced by the loss of contact staining, but not nuclear staining, in jac-1 mutant embryos (d). (e,f) GFP-JAC-1 at cell contacts in a live control embryo (41/41 embryos) and in the cytoplasm in a live hmr-1 embryo (25/25 embryos). (g) HMP-1 immunostaining in a hmp-1(RNAi) embryo. (h) HMP-1 immunostaining in a hmr-1 embryo, where HMP-1 localizes to the cytoplasm (45/45 embryos). (i) Schematic of the jac-1 gene, with exons shown as rectangles and introns as chevrons. Region encoding the Armadillo (Arm) repeats, which contains the peptide used to generate α-JAC-1 antiserum, is indicated. The dashed region indicates the extent of jac-1 deleted in the xn15 allele. Scale bars, 10 μm.