Supplementary Figure 7: Catenin and HMR-1 localization following catenin depletion.

Control embryos are wild-type embryos fed on bacteria containing RNAi empty vector. (a,b) HMP-1-GFP at cell contacts in wild-type and jac-1 embryos. (c) Quantification of HMP-1-GFP contact enrichment in wild-type and jac-1 embryos (wild type n = 12 embryos, jac-1 n = 12). Circles represent individual values and gray lines the mean. HMP-1-GFP levels at contacts in the two genotypes are not significantly different (p = 0.06, Mann–Whitney U test). (d,e) GFP-JAC-1 at cell contacts in a control and hmp-1(RNAi) embryo. (f) Quantification of GFP-JAC-1 contact enrichment in control and hmp-1(RNAi) embryos. (control n = 13 embryos, hmp-1(RNAi) n = 12). GFP-JAC-1 levels at contacts in the two genotypes are not significantly different (p = 0.3, Mann–Whitney U test). (g–j) HMR-1-GFP expression in live four-cell embryos of the indicated genotype. (k) Quantification of HMR-1-GFP levels at cell contacts in embryos of the indicated genotype (control n = 18 embryos, hmp-1(RNAi) n = 33, jac-1 n = 17, jac-1 + hmp-1(RNAi)n = 17). None of the mean values are significantly different from control (p > 0.05, Mann–Whitney U test). Control samples and hmp-1(RNAi) embryos were pooled from two independent experiments. (l,m) Endogenous HMR-1 immunostaining at cell contacts in control (91/91 embryos) and jac-1(xn15) + hmp-1(RNAi) (133/133 embryos). Scale bars, 10 μm.