Supplementary Figure 4: Rescue of pac-1 polarity defects by GFP-PAC-1ΔPH. | Nature Cell Biology

Supplementary Figure 4: Rescue of pac-1 polarity defects by GFP-PAC-1ΔPH.

From: An instructive role for C. elegans E-cadherin in translating cell contact cues into cortical polarity

Supplementary Figure 4

(a) Wild-type, (b) pac-1(xn6), and (c) pac-1(xn6); GFP-PAC-1ΔPH 6–8 cell embryos stained for PAR-6. (d) PAR-6 asymmetry is lost in pac-1 mutants but is rescued by expression of GFP-PAC-1ΔPH. PAR-6 asymmetry was quantified by determining the polarity index, defined as the ratio of PAR-6 levels at contact-free surfaces versus half of the PAR-6 level at cell contacts (see Methods). Circles represent individual data points and the gray line indicates the average (wild type n = 8 embryos, pac-1 n = 10, pac-1; GFP-PAC-1ΔPHn = 8). Measured by Mann–Whitney U-test, pac-1(xn6) embryos have a polarity index significantly lower than wild type (∗∗∗p < 0.001) and pac-1(xn6); GFP-PAC-1ΔPH (∗∗∗p < 0.001). The polarity index of pac-1(xn6); GFP-PAC-1ΔPH is marginally significantly different from wild type (∗p < 0.05). Samples were pooled from two independent experiments. Scale bars, 10 μm.

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