Supplementary Figure 3: Nuclear CLK-1/COQ7 regulates ROS metabolic gene expression. | Nature Cell Biology

Supplementary Figure 3: Nuclear CLK-1/COQ7 regulates ROS metabolic gene expression.

From: A nuclear role for the respiratory enzyme CLK-1 in regulating mitochondrial stress responses and longevity

Supplementary Figure 3

(a) Nuclear CLK-1 regulates the expression of genes involved in ROS metabolism. qPCR analysis of transcripts from ROS-sensitive retrograde genes (mean values from 3 reactions per condition in n = 3 independent experiments; error bars, s.e.m. ∗P < 0.05 for clk-1(−) compared to other strains). (b) CLK-1nuc(+) rescues the increased transcript levels of genes known to be responsive to ROS (sod-2 and skn-1) in clk-1 null worms (mean values from 3 reactions per condition in n = 3 independent experiments; error bars, s.e.m. ∗P < 0.05 for clk-1(−) compared to other strains). (c) Human homologues of these genes, SOD2 and NRF2, and the NRF2 target gene HMOX1 are increased in cells that have lost nuclear COQ7 (R28A) (mean values from 3 reactions per condition for n = 4 independent experiments; error bars, s.e.m. ∗P < 0.05). (d) Quantification of immunoblots for GLS2, WWOX and HTRA2 proteins (mean values from n = 3 independent experiments; error bars, s.e.m. ∗P < 0.05,∗P < 0.005). Representative blots are shown in Fig. 4i.

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