Supplementary Figure 4: Nuclear CLK-1/COQ7 suppresses the expression of UPR^mt genes.

(a) qPCR analysis of UPR^mt genes in CLK-1 transgenic worm strains relative to N2 (mean values from 3 reactions per condition in n = 3 independent experiments; error bars, s.e.m. n.s., no significant difference; ^∗P < 0.05). The increase in expression of hsp-6, hsp-60 and spg-7 in clk-1(−) worms was abrogated by expression of either CLK-1^wt or CLK-1^nuc(+). mRNA levels of the endoplasmic reticulum UPR (UPR^ER)-regulated gene hsp-4 were not changed in any of the worm strains. (b) qPCR of analysis of transcripts of UPR^mt genes and UPR^ER genes in WT and R28A expressing HEK293 cells (mean values from 3 reactions per condition in n = 4 independent experiments; error bars, s.e.m. ^∗P < 0.05.^∗∗P < 0.005). Heatmap of this data is shown in Fig. 6c. (c) The ratio of COXIV to MTCO1 protein levels in WT and R28A expressing cells quantified from n = 3 independent immunoblots (error bars, s.e.m. n.s., no significant difference). See Fig. 6d for representative immunoblot.