Supplementary Figure 8: Inhibition of mTORC1 mediates mitochondrial remodelling.
From: Autophagy and mTORC1 regulate the stochastic phase of somatic cell reprogramming
(a) Western blotting for MTOR and ATK-mTORC1 signalling in early reprogramming of 2° system (+ DOX). (b) Western blotting for mTORC1 activity in MEFs and ESCs. (c) Western blotting shows the change of mTORC1 signalling pathway in p53 KO TTFs in early reprogramming. Ctrl, 4XFlag. (d) qPCR analysis for Mtor in MEFs transduced with indicated factors on day 1. (e) Western blotting for MTOR, LC3B and p62 in MEFs transduced with the indicated factors on day 1 (−/+Baf). (f) qPCR analysis of genes responsible for mitochondrial biogenesis. (g) FACS analysis shows the effect of Tsc2 shRNAs on cell size. (h) qPCR analysis to confirm the expression of the indicated genes in 4F reprogramming on day 5. Data are plotted from 1 experiment with 3 technical replicates. (i) qPCR analysis of genes responsible for mitochondrial biogenesis in MEFs transduced with 4F and the indicated genes on day 5. (j) FACS analysis for cell size in MEFs transduced with indicated genes on day 5. (k) FACS analysis cell proliferation in MEFs transduced with indicated genes on day 5. Data shown are the mean of 3 replicates and are from 1 of 2 representative experiments (d,k). Means ± s.e.m. from n = 3 (f,g,i) and 4 (j) independent experiments with 3 technical replicates are shown. Source data are provided in Supplementary Table 4. Uncropped images of blots are shown in Supplementary Fig. 6.
