Supplementary Figure 5: Taqman expression analysis of all RasGRP family members in colorectal cancer cell lines.
From: RasGRP1 opposes proliferative EGFR–SOS1–Ras signals and restricts intestinal epithelial cell growth
(a) Real-time PCR of RasGRP1 mRNA level in seven selected colorectal (CRC) cell lines as shown Supplementary Table 1. WiDr was used as an arbitrary reference and set at 1.0. All data are shown as fold difference ± s.e.m. and compared to the value of 1.0 in WiDr and data is plotted from n = 6 independent RNA extractions for each cell type. (b) Same data sets for WiDr and SW403 as in Supplementary Fig. 3a but depicted on a different scale to highlight low expression of RasGRP1 in WiDr and nearly complete absence of RasGRP1 in SW403 CRC cells. WiDr was used as an arbitrary reference and set at 1.0. All data are shown as fold difference ± s.e.m. and compared to the value of 1.0 in WiDr and data is plotted from n = 6 independent RNA extractions for each cell type. (c–e) RasGRP2- (c), RasGRP3- (d) and RasGRP4- (e) mRNA expression levels in the indicated seven CRC cell lines. WiDr was used as an arbitrary reference and set at 1.0. All data are shown as fold difference ± s.e.m. and compared to the value of 1.0 in WiDr and data is plotted from n = 6 independent RNA extractions for each cell type.
