Supplementary Figure 8: Analysis of ApcMin/+ :Rasgrp1-/- mice.
From: RasGRP1 opposes proliferative EGFR–SOS1–Ras signals and restricts intestinal epithelial cell growth
(a) Representative H&E images from colonic tumours from ApcMin/+ and ApcMin/+ :Rasgrp1-/- mice. Top panels (same as in Fig. 8) show the entire tumours and bottom panel show the same tumours at larger magnification. Top panels have a scale of 150 μm, whereas the bottom panels scale is 30 μm. (b) Representative colonic tumour sections from ApcMin/+ and ApcMin/+ :Rasgrp1-/- mice that were stained for BrdU (in brown, 2 h in vivo BrdU labeling). Scale 150 μm. Details within the traced squares are shown at higher magnification. (c) Representative tumour sections stained for cleaved caspase-3 (in brown) to reveal apoptotic cells in ApcMin/+ and ApcMin/+ :Rasgrp1-/- mice with an inset showing rare, positive cells at higher magnification. Arrows indicate the rare cleaved caspase-3 positive cells. Scale 150 μm. Details within the traced squares are shown at higher magnification.
