Supplementary Figure 3: Loss of Rasgrp1 alleles exacerbates the serrated dysplasia and hyperproliferation driven by KRasG12D.
From: RasGRP1 opposes proliferative EGFR–SOS1–Ras signals and restricts intestinal epithelial cell growth
(a) Higher magnifications of the distal colon of the indicated three mouse models depicted in Fig. 3b. Sections have been immunostained for Ki67 expression in brown. Scale bar indicates 100 μm. (b) Quantification of counted Ki67-positive cells in open crypts of the distal colon in mice from the indicated genotypes. Data are mean ± s.e.m. determined from n = 150 crypts that were pooled from three mice for each genotype. ∗∗∗p < 0.0001 (One way Anova, Bonferroni’s multiple comparison test). (c) Aberrant serration in the distal colon in KRasG12DRasgrp1WT/- heterozygous mice. H&E staining of a representative section of the distal colon of KRasG12DRasgrp1WT/- mice. Scale bar indicates 100 μm.
