Supplementary Figure 10: Puncta formation and colocalization analysis of p62 in comparison with LC3 which is produced from RFP-GFP-LC3. | Nature Cell Biology

Supplementary Figure 10: Puncta formation and colocalization analysis of p62 in comparison with LC3 which is produced from RFP-GFP-LC3.

From: Amino-terminal arginylation targets endoplasmic reticulum chaperone BiP for autophagy through p62 binding

Supplementary Figure 10

HeLa cells expressing RFP-GFP-LC3 were treated with poly(dA:dT) dsDNA, followed by immunofluorescence analysis of acid-resistant RFP-LC3 and acid-sensitive GFP-LC3. Note that most LC3 puncta are positive for both RFP and GFP, indicating that they represent autophagosomes.

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