Supplementary Figure 6: Role of contractility in generating tensions during compaction.
From: Pulsatile cell-autonomous contractility drives compaction in the mouse embryo
a, Immunostaining of Myh9 early and late in the 8-cell stage. Red arrowheads point at cell–medium interfaces, green ones at cell–cell contacts. Scale bar, 10 μm. b, Mean ± s.d. contact/cortex ratio, n = 194 contacting blastomeres pooled from 22 embryos from 2 representative experiments, Pearson correlation R = −0.538 (p < 0.001). c, Phalloidin staining of F-actin early and late in the 8-cell stage. Red arrowheads point at cell–medium interfaces, green ones at cell–cell contacts. d, Mean ± s.d. contact/cortex ratio, 113 embryos from 9 representative experiments, Pearson correlation R = −0.752 (p < 0.001). e, Time-lapse of mTmG expressing embryo. Time post-division (hh:mm), Supplementary Video 3 . f, Contact/cortex ratio of mTmG and LifeAct-GFP over time. Mean ± s.d. of n = 23 contacts pooled from 8 embryos from 3 representative experiments followed every 30 min for 5–8 h. g, Surface tension measurement of DMSO or 1 μM CCD treated embryos. Scale bar, 10 μm. h, Surface tension γcm as a function of the contact angle θi of n = 10 and 42 contacting blastomeres pooled from 2 and 3 embryos treated with DMSO (black) or CCD (yellow) respectively from 1 representative experiment. Pearson correlation R = −0.028 for DMSO and −0.005 for CCD with (p > 0.1). WT (grey) shown in Fig. 1c.
