Supplementary Figure 8: Periodic contractions in different conditions.

a–d, Mean ± s.d. amplitude of periodic contractions as a function of the oscillation period for control 8-cell stage embryos (a–d, in black, n = 23 from 6 representative experiments), 1 μM CCD-treated 8-cell stage embryos (a, in yellow, n = 14 from 2 representative experiments), 4-cell stage embryos (b, in blue, n = 8 from 2 representative experiments), 8-cell stage embryos without zona pellucida (c, No ZP, in purple, n = 6 from 2 representative experiments). mzCdh1^−/− 8-cell stage embryos (d, in green, n = 7 from 3 representative experiments). e, After Fourier analysis, the mean power spectra are analysed for the presence of a distinct peak of amplitude at a specific frequency. Distinct peaks could be observed in 16 out of n = 23 embryos at 8-cell stage, 1 out of n = 8 embryos at 4-cell stage, 0 out of n = 17 embryos at 8-cell stage with 25 μM Bb (−), 0 out of n = 14 embryos at 8-cell stage with 1 μM CCD, 6 out of n = 6 embryos at 8-cell stage without zona pellucida (ZP), 7 out of n = 7 mzCdh1^−/− embryos at 8-cell stage, 7 out of n = 7 blastomeres isolated at the 8-cell stage. f, Periods that could be extracted are plotted as mean ± s.d. for embryos at 8-cell stage after PIV analysis (n = 16) and LifeAct-GFP intensity changes (n = 4), n = 6 embryos at 8-cell stage without zona pellucida (ZP) after PIV analysis, n = 7 mzCdh1^−/− embryos at 8-cell stage after PIV analysis, n = 7 blastomeres isolated at the 8-cell stage after curvature analysis and LifeAct-GFP intensity changes.