Supplementary Figure 2: Blastomeres volume during the 8-cell stage.
From: Pulsatile cell-autonomous contractility drives compaction in the mouse embryo
a, Images of cell volume reconstruction showing 6 cells of an 8-cell stage embryo expressing mTmG (grey) artificially labelled in distinct colours using Bitplane Imaris. Scale bar, 10 μm. b, Volume of blastomeres at the beginning (30 min after the last 4- to 8-cell stage division) and end (30 min before rounding up of the first 8- to 16-cell stage division) of the 8-cell stage. Individual examples from (a) are shown in colour while the whisker plot in black shows the minimum and maximum values, and upper and lower quartiles of n = 32 blastomeres pooled from 4 embryos from 1 representative experiment. p value indicates the result of Student’s t-test.
