Supplementary Figure 10: Phosphorylation of TOR-Dependent Sites S614 and S617 Mediate Decapping of ATG5 T ranscripts, Related to Fig. 4.
From: A conserved mechanism of TOR-dependent RCK-mediated mRNA degradation regulates autophagy
(a) Yeast cells under the indicated conditions expressing either WT Dcp2 or an equivalent protein containing either the S > D mutation or S > A mutation at positions S614 and S617 were assayed for decapping of ATG5 transcripts over 30 min by a PCR assay and visualized by ethidium bromide as in Fig. 3b. (b) Densitometry of n = 3 independent decapping assays performed as in Supplementary Fig. 3a. Bars + / − SD; Student’s t-test, ∗p < 0.05,∗∗p < 0.01,∗∗∗p < 0.001.
