Supplementary Figure 7: Immune gene expression following IEC-Atg7 deletion.

(a,b) The expression of genes associated with cytotoxic CD8⁺ T cells (CD2, CD3γ, CD8α, CD8β, IL15, ZAP70, GZMA, GZMβ, GZMκ, PRF1), T_H1 cells (Stat1, IRF1, IFNγ, TBX21, IL12Rb1), Treg cells (FOXP3, CTLA4, TGFβ1), and T_H17 cells (Il17Rβ, IL23α, ROR_C, IRF4, CCL20, CCR6, STAT3) assessed by qPCR in normal colon (a) and duodenum (b) from Apc^+/−Atg7^−/− compared to Apc^+/− mice. (c) Transcript levels of of CX3CL1, CXCL9, CXCL10 mRNA was analysed by qPCR in the colon and duodenum from Apc^+/− mice or Apc^+/−Atg7^−/− mice. Gene expression levels were normalized to the abundance of 18s rRNA for each sample (mean ± SD, (a–c) n: Apc^+/− = 9 extracts from 9 mice and Apc^+/−Atg7^−/− = 10 extracts from 10 mice, mice were pooled from three independent experiments, ^∗significant differences, (a) ^∗1P = 0.0108, ^∗2P = 0.0257, ^∗3P = 0.0185, ^∗4P = 0.0150, ^∗5P = 0.0180, ^∗6P = 0.0123, ^∗7P = 0.0109, ^∗8 = 0.0200, ^∗9P = 0.0006, ^∗10P = 5.25 × 10⁻⁰⁸, ^∗11P = 2.51 × 10⁻⁰⁷, ^∗12P = 0.0006, ^∗13P = 0.0134, ^∗14P = 0.0009, ^∗15P = 0.0154, ^∗16P = 0.0195; (b) ^∗1P = 0.0366, ^∗2P = 0.0472, ^∗3P = 0.0413, ^∗4P = 0.0313, ^∗5P = 0.0006, ^∗6P = 0.0156, ^∗7P = 0.0055, ^∗8P = 0.0473, ^∗9P = 0.0187, ^∗10P = 0.0294, ^∗11P = 0.0142, ^∗12P = 0.0356, ^∗13P = 0.0002, ^∗14P = 0.0070, ^∗15P = 0.0038, ^∗16P = 0.0006, ^∗17P = 0.0305, ^∗18P = 0.0109 (c)^∗1P = 0.0005, ^∗2P = 0.0278, ^∗3P = 0.0136, ^∗4P = 0.0281, ^∗5P = 0.0338, ^∗6P = 0.0021, two-tailed unpaired t-test).