Supplementary Figure 10: β-catenin signaling in adenoma following IEC-Atg7 deletion.

Representative hematoxylin/eosin staining of colonic adenomas from Apc^+/− and Apc^+/−Atg7^−/− mice. Polyps from Apc^+/−Atg7^−/− mice were smaller than those from Apc^+/− mice. (b,c) Gene expression levels of several β-catenin target genes (Axin2, c-Myc, c-Jun, Sox9) assessed by qPCR of adenomas (Ade) from the indicated genotype relative to non-tumoral colon of Apc^+/− mice (NT). Gene expression levels were normalized to the abundance of 18s rRNA for each sample (mean ± SD., n: NT Apc^+/− = 8 extracts from 8 mice; Ade Apc^+/− = 8 tumors from 8 mice and Ade Apc^+/−Atg7^−/− = 8 tumors from 8 mice, mice were pooled from two independent, ^∗significant differences, (b) colon, ^∗1P = 0.0038, ^∗2P = 0.0042, ^∗3P = 0.0032, ^∗4P = 0.0025, ^∗5P = 0.0047, ^∗6P = 0.0485, ^∗7P = 0.0466, ^∗8P = 0.0472, (c) duodenum ^∗1P = 0.0091, ^∗2P = 0.0192, ^∗3P = 0.0086, ^∗4P = 0.0082, ^∗5P = 0.0037, ^∗6P = 0.0008, ^∗7P = 0.0384, ^∗8P = 0.0478 two-tailed unpaired t-test).