Supplementary Figure 1: IR induces the association of FH with chromatin.

(a) U2OS cells synchronized by thymidine double block (2 mM) underwent no release (G₁ phase) or release for 2 h (S phase) or 6 h (G₂ phase). These cells were then exposed to IR (10 Gy) and analysed by an immunoblotting assay with the indicated antibodies and flow cytometry. Data represent one out of 3 experiments. (b) GSC11 cells that were synchronized by thymidine double block (2 mM) were not released (G₁ phase) or were released for 2 h (S phase) or 6 h (G₂ phase). These cells then underwent IR (10 Gy) and were harvested at the indicated time points. Chromatin extracts were prepared. CENP-A was used as a control for chromatin-associated proteins. Immunoblotting analyses were performed with the indicated antibodies. Data represent one out of 3 experiments. (c) Thymidine double block-synchronized U2OS cells expressing Flag-FH were exposed to IR (10 Gy). Chromatin extracts were subjected to immunoprecipitation with an anti-FH antibody and analysed by mass spectrometry. FH-interacting histone proteins in the cells with or without IR identified by mass spectrometry were shown.